| Product Name | SCF, 26-189, Human (Bioactivity Validated) |
|---|---|
| Description | Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Synonyms | Stem cell factor, KITLG, Kitl, KL-1, MGF, SF, SHEP7 |
| Host | E. coli |
| Molecular Weight |
32.0kDa (284aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 50mM MES (pH5.5) containing 10% glycerol, 100mM NaCl, 5mM CaCl2. |
| Concentration | 0.25mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732. Okada S, et al. (1992). Nippon Rinsho, 50, 1872. |
| Background | Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Supplier | ARP |
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