| Product Name | SH3BGRL, 1-114aa, Human |
|---|---|
| Description | SH3BGRL, also known as SH3 domain-binding glutamic acid-rich-like protein, is a member of the human SH3BGR family. SH3BGRL gene located to chromosome Xq13.3 encodes for a small protein of 114 amino acids, which is apparently widely expressed in many tissues including liver and blood. SH3BGRL protein features a prolinerich sequence (PLPPQIF), which contains both the SH3 binding (PXXP)3 and the Homer EVH1 binding (PPXXF)4 motif. This proline-rich region is highly conserved in human SH3BGR family and was expected to be functionally important. Recombinant human SH3BGRL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | SH3 domain-binding glutamic acid-rich-like protein, SH3BGR |
| Host | E. coli |
| Molecular Weight | 38.6kDa (354aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMVIRVY IASSSGSTAI KKKQQDVLGF LEANKIGFEE KDIAANEENR KWMRENVPEN SRPATGYPLP PQIFNESQYR GDYDAFFEAR ENNAVYAFLG LTAPPGSKEA EVQAKQQA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer, pH 7.5, 10% glycerol, 1mM DTT, 200mM NaCl |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Egeo A., et al. (1998) Biochem. Biophys. Res. Commun. 247:302-306 Yin L., et al. (2006) Proteins. 61:213-216 |
| Background | SH3BGRL, also known as SH3 domain-binding glutamic acid-rich-like protein, is a member of the human SH3BGR family. SH3BGRL gene located to chromosome Xq13.3 encodes for a small protein of 114 amino acids, which is apparently widely expressed in many tissues including liver and blood. SH3BGRL protein features a prolinerich sequence (PLPPQIF), which contains both the SH3 binding (PXXP)3 and the Homer EVH1 binding (PPXXF)4 motif. This proline-rich region is highly conserved in human SH3BGR family and was expected to be functionally important. Recombinant human SH3BGRL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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