| Product Name | SPG21, 1-308aa, Human, Recombinant |
|---|---|
| Description | Spastic paraplegia 21(SPG21), also known as Maspardin, binds to the hydrophobic C-terminal amino acids of CD4 and may play a role as a negative regulatory factor in CD4-depenent T-cell activation. This protein is widely expressed in various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in SPG21 cause Mast syndrome, an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum and white matter abnormalities. Recombinant human SPG21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | ACP33, BM-019, GL010, Maspardin, MAST, Spastic paraplegia 21 isoform a Acid cluster protein 33, BM019, Spastic paraplegia 21 autosomal recessive Mast syndrome protein, SPG21. |
| Host | E. coli |
| Molecular Weight | 14.2 kDa (122aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS LGISQEEQ |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Simpson MA., et al. (2003). Am J Hum Genet. 73(5):1147-56. Zeitlmann L., et al. (2001). J Biol Chem. 276(12):9123-32. |
| Background | Spastic paraplegia 21(SPG21), also known as Maspardin, binds to the hydrophobic C-terminal amino acids of CD4 and may play a role as a negative regulatory factor in CD4-depenent T-cell activation. This protein is widely expressed in various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in SPG21 cause Mast syndrome, an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum and white matter abnormalities. Recombinant human SPG21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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