| Product Name | ST6GAL1, 27-406aa, Human (Denatured) |
|---|---|
| Description | ST6GAL1 also known as Beta-galactoside alpha-2,6-sialyltransferase isoform a, is a member of glycosyltransferase family 29. This protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. ST6GAL1, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. Recombinant human ST6GAL1, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Beta-galactoside alpha-26-sialyltransferase 1 isoform a, Beta-galactoside alpha-2,6-sialyltransferase 1 isoform a, SIAT1, ST6GalI, ST6N |
| Host | E. coli |
| Molecular Weight |
76.5kDa (692aa) 70-100KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSKEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIW KNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREID DHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPF YILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLN QGTDEDIYLL GKATLPGFRT IHC |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in phosphate buffered saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Kuhn B.,et al. (2013) Acta Crystallogr. D Biol. Crystallogr. 69 (PT 9), 1826-1838 |
| Background | ST6GAL1 also known as Beta-galactoside alpha-2,6-sialyltransferase isoform a, is a member of glycosyltransferase family 29. This protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. ST6GAL1, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. Recombinant human ST6GAL1, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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