| Product Name | STIM1, 23-213aa, Human, CaM tagged, Recombinant |
|---|---|
| Description | Stromal interaction molecule 1 (STIM1) is a transmembrane protein that is essential for store-operated Ca(2+) entry, a process of extracellular Ca(2+) influx in response to the depletion of Ca(2+) stores in the endoplasmic reticulum (ER). STIM1 localizes predominantly to the ER; upon Ca(2+) release from the ER, STIM1 translocates to the ER-plasma membrane junctions and activates Ca(2+) channels. Recombinant Calmodulin tagged STIM was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | GOK, D11S4896E, Stromal interaction molecule 1, STIM1, Stromal interaction molecule 1 D11S4896E, SIM, STIM 1, Stim1 stromal interaction molecule 1. |
| Host | E.coli |
| Molecular Weight | 23 kDa (206 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 25mM Tris pH 7.5, 1mM DTT, 2mM EDTA, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Varnai P., et al. (2007) J. Biol. Chem . 282(40):29678-90. Grigoriev I., et al. (2008) Curr Biol. 18(3):177-82. |
| Supplier | ARP |
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