| Product Name | SULT2A1, 1-285aa, Human |
|---|---|
| Description | SULT2A1, also known dehydroepiandrosterone sulphotransferase, belongs to the sulfotransferase family. This protein is mainly expressed in liver and adrenal tissues, and to a lesser extent in kidney. It catalyzes the 3'-phosphoadenosine 5'-phosphosulfate-dependent sulfation of a wide variety of steroids in human liver and adrenal tissues, and is also responsible for most of the sulfation of bile acids in human liver. Recombinant human SULT2A1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Bile salt sulfotransferase, DHEA-ST, DHEAS, HST, hSTa, ST2, ST2A1, ST2A3 |
| Host | E. coli |
| Molecular Weight | 13.9 kDa (121aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MSDDFLWFEG IAFPTMGFRS ETLRKVRDEF VIRDEDVIIL TYPKSGTNWL AEILCLMHSK GDAKWIQSVP IWERSPWVES EIGYTALSET ESPRLFSSHL PIQLFPKSFF SSKAKVIYLM RNPRDVLVSG YFFWKNMKFI KKPKSWEEYF EWFCQGTVLY GSWFDHIHGW MPMREEKNFL LLSYEELKQD TGRTIEKICQ FLGKTLEPEE LNLILKNSSF QSMKENKMSN YSLLSVDYVV DKAQLLRKGV SGDWKNHFTV AQAEDFDKLF QEKMADLPRE LFPWE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.2M NaCl. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Otterness D.M. et al. (1992) Mol. Pharmacol. 41: 865-872. Comer K.A. et al. (1993) Biochem. J. 289: 233-240. |
| Background | SULT2A1, also known dehydroepiandrosterone sulphotransferase, belongs to the sulfotransferase family. This protein is mainly expressed in liver and adrenal tissues, and to a lesser extent in kidney. It catalyzes the 3'-phosphoadenosine 5'-phosphosulfate-dependent sulfation of a wide variety of steroids in human liver and adrenal tissues, and is also responsible for most of the sulfation of bile acids in human liver. Recombinant human SULT2A1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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