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|Product Name||14-3-3 zeta, 1-245aa, Human, Recombinant|
|Background||The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||YWHAZ, KCIP-1, MGC111427, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, 14-3-3 zeta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide 14 3 3 protein zeta/delta, KCIP1, Phospholipase A2, Protein kinase C inhibitor protein 1, Tyr-3/Trp-5 monooxygenase Activation protein, Zeta, Tyr3/Trp5 monooxygenase Activation protein, Zeta|
|Amino Acid Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN|
|Molecular Weight||28 kDa (246 aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris pH 8.0, 1mM EDTA, 50mM NaCl|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_ 663723|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Gannon-Murakami L., et al. (2002) J Biol Chem. 277(26): 23116-23122
Li FQ., et al. (2008) J Cell Biol. 181(7):1141-54
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