| Product Name | ACY3, 1-319aa, Human |
|---|---|
| Description | Aspartoacylase 3, also known as ACY3, is member of the Aspartoacylase subfamily. It plays an important role in deacetylating mercapturic acids in kidney proximal tubules. ACY3 localizes to the cytoplasm of S2 and S3 proximal tubules and to the apical domain of S1 proximal tubules. ACY3 protein is also expressed at low levels in stomach, testis, heart, brain, lung and liver, and may function as an HCV (Hepatitis C virus) core binding protein. Recombinant human ACY3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3 |
| Host | E.coli |
| Molecular Weight | 42.7kDa (387aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMCSLPVP REPLRRVAVT GGTHGNEMSG VYLARHWLHA PAELQRASFS AVPVLANPAA TSGCRRYVDH DLNRTFTSSF LNSRPTPDDP YEVTRARELN QLLGPKASGQ AFDFVLDLHN TTANMGTCLI AKSSHEVFAM HLCRHLQLQY PELSCQVFLY QRSGEESYNL DSVAKNGLGL ELGPQPQGVL RADIFSRMRT LVATVLDFIE LFNQGTAFPA FEMEAYRPVG VVDFPRTEAG HLAGTVHPQL QDRDFQPLQP GAPIFQMFSG EDLLYEGEST VYPVFINEAA YYEKGVAFVQ TEKFTFTVPA MPALTPAPSP AS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Chen Y R., et al. (2009) J Gastroenterol Hepatol. 24(7):1300-4. Pushkin A., et al. (2004). Am J Physiol Cell Physiol. 286(4):C848-56. |
| Supplier | ARP |
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