AIF1, 1-147aa, Human

Proteins, Recombinant
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Product Name AIF1, 1-147aa, Human
Background AIF1 (Allograft inflammatory factor 1) is induced by cytokines and interferon. This protein is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. AIF1 is a cytoplasm, calcium-binding protein whose expression in transplanted human hearts correlates with rejection and development of coronary artery vasculopathy (CAV). Recombinant human AIF1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Allograft inflammatory factor 1, AIF-1, IBA1, IRT-1, IRT1
Species Human
Molecular Weight 29.1 kDa (281aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 150mM NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001614
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Kelemen S.E., et al (2005) Am. J. Pathol. 167:619-626
Schulze J.O., et al. (2008) FEBS J. 275:4627-4640

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