Anti-Ep-CAM (Human Epithelium Antigen-HEA), Monoclonal Antibody, FITC
| Product Name | Anti-Ep-CAM (Human Epithelium Antigen-HEA), Monoclonal Antibody, FITC |
|---|---|
| Description | Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. |
| Clone | HEA125 |
| Immunogen | HT-29 colon carcinoma cell line |
| Isotype | IgG1 |
| Specificity | Reactivities on cultured cell line: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma lines (HT-29, WiDr, SW1116). Polypeptide reacting: MR 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM). |
| Reactivity | Human |
| Applications | IF |
| Form | Purified (Protein A), in PBS containing 1% BSA and 0.1% sodium-azide |
| Storage | 2-8C for immediate use, or at -20C (aliquot) |
| References | Edwards, P.A.W. (1985) Br. J. Cancer 51: 149-160Hastka, J.& Pfiester, P. (1988). Path. 9: 245-247Momburg, F. et al. (1985) Verh. Dtsch. Ges. Path. 69: 627ppMomburg, F. et al (1987) Cancer Res. 47: 2883-2891Joplin, R et al. (1990) J. Pathol. 162: 255-260Racila, E. et al. (1998) Proc. Natl. Acad. Sci USA 92: 4589-4594Balzar, M. et al. (1999) L. Mol. Med. 77: 699-712 |
| Supplier | ARP |
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