Anti-Monkey secretory component (free and bound), Biotin Polyclonal Antibody
| Product Name | Anti-Monkey secretory component (free and bound), Biotin Polyclonal Antibody |
|---|---|
| Description | Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The conjugate reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in monkey body fluids. As a second step an avidin or streptavidin conjugate of the userÍs choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:1,000. |
| Host | Goat |
| Clone | Polyclonal |
| Immunogen | Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimmer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization. FreundÍs complete adjuvant is used in the first step of the immunization procedure. |
| Reactivity | Primate |
| Applications | ELISA, DOT, ICC, IHC, WB |
| Storage | 2-8C for immediate use, or at -20C (aliquot). Avoid repeated freezing and thawing |
| Supplier | ARP |
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