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|Product Name||ASF1B, 1-202aa, Human|
|Background||ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. This protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Recombinant human ASF1B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II|
|Amino Acid Sequence||MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH|
|Molecular Weight||10.8 kDa (97aa), confirmed by MALDI-TOF|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol|
|Purity||> 85% by SDS-PAGE|
|NCBI Accession #||NP_060624|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Corpet,A., et al. (2011) EMBO J. 30 (3), 480-493
Jasencakova,Z., et al. (2010) Mol. Cell 37 (5), 736-743
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