CERT, 347-598aa, Human

Catalog
01-1846
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Proteins, Recombinant
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Product Name CERT, 347-598aa, Human
Background CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms COL4A3BP, CERTL, GPBP, STARD11, Collagen, type IV, alpha 3 binding protein Alpha 3 type IV collagen binding protein, Ceramide transporter, CERT, Cert protein, Collagen type IV alpha 3 (Goodpasture antigen) binding protein, Collagen type IV alpha 3 binding protein, FLJ20597, Goodpasture antigen binding protein, Lipid transfer protein CERTL, Procollagen type IV alpha 3 (Goodpasture antigen) binding protein, StAR related lipid transfer protein 11, StARD 11, START domain containing 11, START domain containing protein 11,
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF
Molecular Weight 40.4 kDa (377aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_112729
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Turner N., et al. (1992) J Clin Invest 89(2):592-601.

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