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|Product Name||DUSP3, 1-185aa, Human|
|Background||DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DUSP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Dual specificity protein phosphatase 3, VHR|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP|
|Molecular Weight||44.5 kDa (393aa)|
|Concentration||0.25 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT,0.1mM PMSF|
|Purity||> 95 % by SDS-PAGE|
|NCBI Accession #||NP_004081|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Todd JL., et al. (1999) J Biol Chem. 274(19):13271-80.
Keyse SM., et al. (1995) Biochim Biophys Acta. 1265(2-3):152-60.
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