| Product Name | HPRT1, 1-218aa, Human |
|---|---|
| Description | Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1. |
| Host | E.coli |
| Molecular Weight | 10.4 kDa (93aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline pH7.4 containing 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Hladnik U., et al. (2008) Arch Neurol. 65(9):1240-3. Sculley DG., et al. (1992) Hum Genet. 90(3):195-207191. |
| Supplier | ARP |
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