| Product Name | HRSP12, 1-137aa, Human |
|---|---|
| Description | HRSP12, also called ribonuclease UK114, is an endoribonuclease found predominantly in human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. This protein cleaves phosphodiester bonds only in single-stranded RNA. It may be an important biomarker for heptatic carcinoma. Recombinant human HRSP12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Ribonuclease UK114, p14.5, PSP, UK114 |
| Host | E.coli |
| Molecular Weight | 16.2 kDa (137aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL |
| Tag | T7 tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl. |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Schmiedeknecht G., et al. (1996). Eur J Biochem. 242(2):339-51. Manjasetty BA, et al. (2004). Proteins 54(4):797-800 |
| Supplier | ARP |
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