MRPL1, 51-325aa, Human

Catalog
01-P2052
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Proteins, Recombinant
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Product Name MRPL1, 51-325aa, Human
Background 39S ribosomal protein L1, mitochondrial precursor, also known as MRPL1, is a 338 amino acid mitochondrial protein that exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRPL1 exists as two isoforms produced by alternative splicing. Isoform one of MRPL1 is ubiquitously expressed while isoform two is specifically expressed in heart. Recombinant human MRPL1 proteinto His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms 39S ribosomal protein L1, mitochondrial precursor, BM022, L1MT, MRP-L1
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSKKTKKGA KEKTPDEKKD EIEKIKAYPY MEGEPEDDVY LKRLYPRQIY EVEKAVHLLK KFQILDFTSP KQSVYLDLTL DMALGKKKNV EPFTSVLSLP YPFASEINKV AVFTENASEV KIAEENGAAF AGGTSLIQKI WDDEIVADFY VAVPEIMPEL NRLRKKLNKK YPKLSRNSIG RDIPKMLELF KNGHEIKVDE ERENFLQTKI ATLDMSSDQI AANLQAVINE VCRHRPLNLG PFVVRAFLRS STSEGLLLKI DPLLPKEVKN EESEKEDA
Molecular Weight 25.9 kDa (239aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol
Purity > 80% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_064621
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Spirina O., et al. (2000) Gene. 261:229-234
Kenmochi N., et al. (2001) Genomics. 77:65-70.

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