NAP1L4, 1-375aa, Human

Catalog
01-P2317
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Proteins, Recombinant
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Product Name NAP1L4, 1-375aa, Human
Background NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Recombinant human NAP1L4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Nucleosome assembly protein 1-like 4, hNAP2, NAP1L4b, NAP2, NAP2L
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV
Molecular Weight 51.3 kDa (455aa)
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005960
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Olsen J.V., et al. (2006) Cell. 127:635-648
Nousiainen M., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396

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