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|Product Name||NFNB, 1-217aa|
|Background||NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV|
|Molecular Weight||36.0 kDa (315aa) confirmed by MALDI-TOF|
|Concentration||1.0 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT, 1mM EDTA|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_415110|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Jarrom D., et al. (2009) Biochemistry. 48(32):7665-72.
Singleton DC., et al. (2007) Cancer Gene Ther. 14(12):953-67.
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