| Product Name | NUDT2, 1-147aa, Human |
|---|---|
| Description | NUDT2 is a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. This protein maintains homeostasis by using water to cleave the metabolite NUDT symmetrically back into its original ATP and AMP molecules. NUDT2 is also active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, but not towards diadenosine triphosphate. NUDT2 is involved in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations. Recombinant NUDT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | bis(5'-nucleosyl)-tetraphosphatase [asymmetrical], APAH1, MGC10404 |
| Host | E.coli |
| Molecular Weight | 15.8 kDa (137aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MALRACGLII FRRCLIPKVD NNAIEFLLLQ ASDGIHHWTP PKGHVEPGED DLETALRETQ EEAGIEAGQL TIIEGFKREL NYVARNKPKT VIYWLAEVKD YDVEIRLSHE HQAYRWLGLE EACQLAQFKE MKAALQEGHQ FLCSIEA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References | Swarbrick JD., et al. (2005) J Biol Chem. 280(9):8471-81. |
| Supplier | ARP |
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