| Product Name | PAFAH1B3, 1-231aa, Human |
|---|---|
| Description | PAFAH1B3 protein belongs to the 'GDSL' lipolytic enzyme family. Acetylhydrolase is catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. PAFAH1B3 is a subunit of the platelet-activating factor cetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex plays an important role during the development of brain. Recombinant human PAFAH1B3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Platelet-activating factor acetylhydrolase IB subunit gamma, PAFAHG, PAF-AH1b alpha 1 subunit |
| Host | E.coli |
| Molecular Weight | 24.1 kDa (216aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMSGEENP ASKPTPVQDV QGDGRWMSLH HRFVADSKDK EPEVVFIGDS LVQLMHQCEI WRELFSPLHA LNFGIGGDGT QHVLWRLENG ELEHIRPKIV VVWVGTNNHG HTAEQVTGGI KAIVQLVNER QPQARVVVLG LLPRGQHPNP LREKNRQVNE LVRAALAGHP RAHFLDADPG FVHSDGTISH HDMYDYLHLS RLGYTPVCRA LHSLLLRLLA QDQGQGAPLL EPAP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol,1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Moro F, et al. (1998) Genomics 51 (1): 157–9 TW, Li J, et al. (2001) Protein Eng. 14 (7): 513–9. |
| Supplier | ARP |
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