PSMB4, 46-264aa, Human

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01-2127-4
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Proteins, Recombinant
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Product Name PSMB4, 46-264aa, Human
Background Proteasome subunit beta type-4, also known as PSMB4, belonged to the peptidase T1B family. The 20S Proteasome chamber contains alpha subunits (which are structural) and beta subunits (which are predominantly catalytic). The outer two rings in the proteasome consist of seven alpha subunits each and the inner two rings each consist of seven beta subunits. PSMB4 is a beta subunit of the 20S Proteasome. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMB4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Proteasome subunit beta type-4, HN3, HsN3, PROS-26, PROS26
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE
Molecular Weight 34.1kDa (304aa)
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl, pH8.0, 2M Urea, 20%Glycerol, 2mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002787
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Orlowski M., et al. (1997) Biochemistry. 36:13946-13953.
McCusker D., et al. (1997) Genomics. 45:362-367.

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