RAB34, 1-259aa, Human

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01-RAB34
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Proteins, Recombinant
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Product Name RAB34, 1-259aa, Human
Background RAB34, also known as ras-related protein Rab34, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. This protein is involved in protein transport, specifically the redistribution of lysosomes to the peri-Golgi region. In fibroblasts, this protein was colocalized with actin to the membrane ruffles and membranes of relatively large vesicles adjacent to the ruffles. Recombinant human RAB34 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms Ras-related protein Rab-34, RAB39, RAH
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN SDDSNLYLTA SKKKPTCCP
Molecular Weight 25.5 kDa (235aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Concentration 1 mg/ml (determined by BCA)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_114140
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Sun P. et al. (2003) J Biol Chem. 278 :4063-71.
Novick P. et al. (1993) Cell. 75: 597-601.

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