RAD51B, 1-350aa, Human

Catalog
01-P2465
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Proteins, Recombinant
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Product Name RAD51B, 1-350aa, Human
Background RAD51B is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Recombinant human RAD51B protein, fused to His-tag at N-terminus, was expressed in E.coli .
Synonyms RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA
YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE
SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE
EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI
KEEGLVLQAY GNS
Molecular Weight 35.5kDa (320aa), confirmed by MALDI-TOF
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
Purity >85 % by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002868
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Masson J.Y., Tarsounas M.C. et al. (2001). Genes Dev. 15:3296-3307

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