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|Product Name||RAD51D, 1-328aa, Human|
|Background||RAD51D is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Recombinant human RAD51D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||RAD51 homolog D isoform 1, BROVCA4, R51H3, RAD51L3, TRAD|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T|
|Molecular Weight||44.7 kDa (391aa) confirmed by MALDI-TOF|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl, 1mM DTT|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_002869|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Masson J.Y., et al. (2001) Genes Dev. 15:3296-3307
Liu N., et al. (2002) Nucleic Acids Res. 30:1009-1015
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