Product Name | RPL12, 1-165aa, Human |
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Description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL12 is a ribosomal protein that is a component of the 60S subunit. This protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. RPL12 binds directly to the 26S rRNA. Recombinant human RPL12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | Ribosomal protein L12, L12 |
Host | E.coli |
Molecular Weight |
16.1 kDa (147aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPPKFDP NEIKVVYLRC TGGEVGATSA LAPKIGPLGL SPKKVGDDIA KATGDWKGLR ITVKLTIQNR QAQIEVVPSA SALIIKALKE PPRDRKKQKN IKHSGNITFD EIVNIARQMR HRSLARELSG TIKEILGTAQ SVGCNVDGRH PHDIIDDINS GAVECPAS |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT |
Concentration | 0.5 mg/ml (determined by BCA assay) |
Purity | > 95% by SDS-PAGE |
Storage |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
References |
Rush J., et al. (2005) Nat. Biotechnol. 23:94-101 Daub H., et al. (2008) Mol. Cell. 31:438-448 |
Supplier | ARP |
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