RPL12, 1-165aa, Human

Proteins, Recombinant
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Product Name RPL12, 1-165aa, Human
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL12 is a ribosomal protein that is a component of the 60S subunit. This protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. RPL12 binds directly to the 26S rRNA. Recombinant human RPL12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Ribosomal protein L12, L12
Species Human
Molecular Weight 16.1 kDa (147aa) confirmed by MALDI-TOF
(Molecular size on SDS-PAGE will appear higher)
Concentration 0.5 mg/ml (determined by BCA assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_000967
Host E.coli
Tag His-tag
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Rush J., et al. (2005) Nat. Biotechnol. 23:94-101
Daub H., et al. (2008) Mol. Cell. 31:438-448

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