S100A3, 1-101aa, Human

Catalog
01-2081A3
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Proteins, Recombinant
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Product Name S100A3, 1-101aa, Human
Background S100A3 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A3 binds calcium with low affinity and zinc with high affinity and is believed to play a role in the calcium-dependent differentiation of cuticle cells and in the formation of hair shafts. Recombinant human S100A3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms S100 calcium binding protein A3, S100E
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q
Molecular Weight 24.2kDa (222aa), confirmed by MALDI-TOF
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 20% glycerol, 100mM NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002951
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Fritz G., et al. (1998) Biochim Biophys Acta. 1448(2):264-76.
Kizawa K., et al. (2008) J Biol Chem. 283(8):5004-13.

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