| Product Name | SecB, 1-155aa, Recombinant |
|---|---|
| Description | SecB, a remarkable chaperone involved in protein export, binds diverse ligands rapidly with high affinity and low specificity. It plays a crucial role during protein export via the general secretory pathway by modulating the partitioning of precursors between folding or aggregation and delivery to the membrane-bound translocation apparatus. SecB has the potential to participate in functions outside of export acting as a general nonspecific chaperone to provide buffering capacity of the nonnative state of proteins in the cytosolic pool. Recombinant E.coli SecB protein was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Protein export chaperone, SecB, Protein export protein secB |
| Host | E.coli |
| Molecular Weight | 44.5 kDa (391 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ LADDVYEVVL RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA YCPNILFPYA RECITSMVSR GTFPQLNLAP VNFDALFMNY LQQQAGEGTE EHQDA |
| Tag | His-tag |
| Reactivity | E. coli |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl 20% glycerol, and 1 mM DTT |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Driessen AJ., et al. (2008), Annu Rev Biochem. 77:643-67. Bechtluft P., et al. (2007). Science. 318(5855):1458-61. |
| Supplier | ARP |
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