| Product Name | SIRT2, 1-352aa, Human |
|---|---|
| Description | SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Recombinant human SIRT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | NAD-dependent deacetylase sirtuin-2, SIR2, SIR2L, SIR2L2 |
| Host | E.coli |
| Molecular Weight | 32.4 kDa (306aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARYMQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQEDLVEAH GTFYTSHCVS ASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDIVFFGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EKAGQSDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKELEDLVRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
North B.J., et al. (2003) Mol. Cell 11:437-444 Dryden S.C., et al. (2003) Mol. Cell. Biol. 23:3173-3185 |
| Supplier | ARP |
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