TPMT, 1-245aa, Human, Recombinant

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01-2111
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Proteins, Recombinant
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Product Name TPMT, 1-245aa, Human, Recombinant
Background TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine and azathioprine are used as chemotherapeutic agents. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity. Recombinant human TPMT was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Thiopurine S-methyltransferase, TPMT, Thiopurine S-methyltransferase HGNC:12014, S adenosyl L methionine thiopurine S methyltransferase, Thiopurine methyltransferase, Thiopurine S methyltransferase.
Species Human
Amino Acid Sequence MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLY LLTEK
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 17 kDa (167 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris pH 7.5
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAH05339
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Tai HL., et al. (1997) Proc Natl Acad Sci USA. 94:6444-9.
Odani S., et al.(2001) J Biochem. 129(2):213-9.

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