TRIP10, 260-545aa, Human

Catalog
01-P2477
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Proteins, Recombinant
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Product Name TRIP10, 260-545aa, Human
Background Cdc42-interacting protein 4, also known as TRIP10, belongs to the F-BAR family of proteins. These proteins contain an N-terminal, alpha-helical, region that is hydrophobic and considered to be related to the Bin Amphiphysin Rvs (BAR) protein family. Expressed in a variety of tissues, including kidney, brain, liver, lung, heart and pancreas, TRIP10 is required for the Insulin-dependent translocation of Glut4 to the plasma membrane and is essential for the coordination of membrane tubulation with Actin cytoskeletal reorganization during endocytosis. Recombinant human TRIP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms CIP4, HSTP, STOT, STP
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSDPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKK
NKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE
SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMA
EGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN
Molecular Weight 9.1kDa (84aa) confirmed by MALDI-TOF
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl (pH8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_004231
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Aspenstrom P., et al. (1997) Curr Biol. 7: 479-487.
Holbert S., et al. (2003) Proc Natl Acad Sci USA. 100: 2712-2717.

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