| Product Name | TTC1, 1-292aa, Human |
|---|---|
| Description | TTC1, also known as tetratricopeptide repeat protein 1, is composed of 292 amino acids with three TPR motifs, as a Galpha16-binding protein. The interaction was confirmed both in vitro and in transfected mammalian cells, where TTC1 also binds to several other Galpha proteins. TTC1 was found to interact with Ha-Ras preferentially in its active form. Recombinant human TTC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Tetratricopeptide repeat protein 1, TPR1 |
| Host | E.coli |
| Molecular Weight |
26.0kDa (222aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMGEKSE NCGVPEDLLN GLKVTDTQEA ECAGPPVPDP KNQHSQSKLL RDDEAHLQED QGEEECFHDC SASFEEEPGA DKVENKSNED VNSSELDEEY LIELEKNMSD EEKQKRREES TRLKEEGNEQ FKKGDYIEAE SSYSRALEMC PSCFQKERSI LFSNRAAARM KQDKKEMAIN DCSKAIQLNP SYIRAILRRA ELYEKTDKLD EALEDYKSIL EKDPSIHQAR EACMRLPKQI EERNERLKEE MLGKLKDLGN LVLRPFGLST ENFQIKQDSS TGSYSINFVQ NPNNNR |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 100mM NaCl |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Liu AM., et al. (2011) BMC Struct Biol. 13;11:17 Strausberg RL., et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 26:16899-16903 |
| Supplier | ARP |
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