WARS, 1-471aa, Human

Catalog
01-1880
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Proteins, Recombinant
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Product Name WARS, 1-471aa, Human
Background WARS, also known as tryptophanyl-tRNA synthetase, belongs to the class I tRNA synthetase family. Two forms of tryptophanyl tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. It also regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Recombinant human WARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q
Molecular Weight 45.8kDa (415aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_776049
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Nagano K., et al. (2004) Oncogene. 23(9):1693-703.

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