Chicken Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit
| Product Name | Chicken Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit |
|---|---|
| Description | Chicken Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken DMRT1. No significant cross-reactivity or interference between Chicken DMRT1 and analogues was observed. DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes. This Chicken Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT1 in samples. An antibody specific for DMRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DMRT1, DMT1, DM domain expressed in testis 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes. |
| Supplier | Abbkine |
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