| Product Name | Bovine Cytochrome b (MT-CYB) ELISA Kit |
|---|---|
| Description | Bovine Cytochrome b (MT-CYB) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MT-CYB. No significant cross-reactivity or interference between Bovine MT-CYB and analogues was observed. Mutations in the mitochondrial DNA cytochrome b gene (MTCYB) have been commonly associated with isolated mitochondrial myopathy and exercise intolerance, rarely with multisystem disorders, and only once with a parkinsonism/mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes (MELAS) overlap syndrome. Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder). Defects in MT-CYB are the cause of cardiomyopathy infantile histiocytoid (CMIH). Defects in MT-CYB contribute to Leber hereditary optic neuropathy (LHON). This Bovine Cytochrome b (MT-CYB) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-CYB in samples. An antibody specific for MT-CYB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-CYB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-CYB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-CYB bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MT-CYB, MTCYB, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 3283889 |
| Storage | 2-8°C |
| Background | Mutations in the mitochondrial DNA cytochrome b gene (MTCYB) have been commonly associated with isolated mitochondrial myopathy and exercise intolerance, rarely with multisystem disorders, and only once with a parkinsonism/mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes (MELAS) overlap syndrome. Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder). Defects in MT-CYB are the cause of cardiomyopathy infantile histiocytoid (CMIH). Defects in MT-CYB contribute to Leber hereditary optic neuropathy (LHON). |
| Supplier | Abbkine |
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