| Product Name | Bovine Insulin-like peptide INSL6 (INSL6) ELISA Kit |
|---|---|
| Description | INSL6 contains a classical signature of the insulin superfamily and is significantly similar to relaxin and relaxin-like factor. This gene is preferentially expressed in testis. Its expression in testis is restricted to interstitial cells surrounding seminiferous tubules, which suggests a role in sperm development and fertilization. The deduced 213-amino acid protein, which shares 45% sequence identity with the mouse protein, contains the classic B-C-A domain configuration, including the 6 cysteines in the B and A domains, of the insulin/relaxin family proteins. Northern blot analysis revealed expression of a 1.2-kb transcript confined to testis. Immunohistochemical analysis demonstrated expression restricted to interstitial cells in mouse testis. Bovine Insulin-like peptide INSL6 (INSL6) ELISA Kit employs a two-site sandwich ELISA to quantitate INSL6 in samples. An antibody specific for INSL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL6 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Bovine INSL6 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of INSL6 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | INSL6, RIF1, insulin-like peptide 5, relaxin/insulin-like factor 1 |
| Method | Sandwich ELISA |
| Detection Range | 5 ng/L-80 ng/L |
| Sensitivity | 0.5 ng/L |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 774023 |
| Storage | 2-8°C |
| Background |
INSL6 contains a classical signature of the insulin superfamily and is significantly similar to relaxin and relaxin-like factor. This gene is preferentially expressed in testis. Its expression in testis is restricted to interstitial cells surrounding seminiferous tubules, which suggests a role in sperm development and fertilization. The deduced 213-amino acid protein, which shares 45% sequence identity with the mouse protein, contains the classic B-C-A domain configuration, including the 6 cysteines in the B and A domains, of the insulin/relaxin family proteins. Northern blot analysis revealed expression of a 1.2-kb transcript confined to testis. Immunohistochemical analysis demonstrated expression restricted to interstitial cells in mouse testis. |
| Supplier | Abbkine |
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