| Product Name | Bovine Kinetochore protein Spc25 (SPC25) ELISA Kit |
|---|---|
| Description | Bovine Kinetochore protein Spc25 (SPC25) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine SPC25. No significant cross-reactivity or interference between Bovine SPC25 and analogues was observed. Kinetochore protein Spc25 (SPC25) acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. This Bovine Kinetochore protein Spc25 (SPC25) ELISA Kit employs a two-site sandwich ELISA to quantitate SPC25 in samples. An antibody specific for SPC25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPC25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPC25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPC25 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SPC25, AD024, MGC22228, SPBC25, 2600017H08Rik, kinetochore protein Spc25, spindle pole body component 25, spindle pole body component 25 homolog |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 505011 |
| Storage | 2-8°C |
| Background | Kinetochore protein Spc25 (SPC25) acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules |
| Supplier | Abbkine |
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