| Product Name | Bovine Metallothionein (MT) ELISA Kit |
|---|---|
| Description | Bovine Metallothionein (MT) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MT. No significant cross-reactivity or interference between Bovine MT and analogues was observed. Metallothionein (MT) is a family of cysteine-rich, low molecular weight (MW ranging from 500 to 14000 Da) proteins. They are localized to the membrane of the Golgi apparatus. MTs have the capacity to bind both physiological (such as zinc, copper, selenium) and xenobiotic (such as cadmium, mercury, silver, arsenic) heavy metals through the thiol group of its cysteine residues, which represent nearly 30% of its constituent amino acid residues. This Bovine Metallothionein (MT) ELISA Kit employs a two-site sandwich ELISA to quantitate MT in samples. An antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MT |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Metallothionein (MT) is a family of cysteine-rich, low molecular weight (MW ranging from 500 to 14000 Da) proteins. They are localized to the membrane of the Golgi apparatus. MTs have the capacity to bind both physiological (such as zinc, copper, selenium) and xenobiotic (such as cadmium, mercury, silver, arsenic) heavy metals through the thiol group of its cysteine residues, which represent nearly 30% of its constituent amino acid residues. |
| Supplier | Abbkine |
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