Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit
| Product Name | Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit |
|---|---|
| Description | Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MYLK. No significant cross-reactivity or interference between Bovine MYLK and analogues was observed. Myosin light chain kinase, smooth muscle (MYLK), a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. MYLK encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. This Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit employs a two-site sandwich ELISA to quantitate MYLK in samples. An antibody specific for MYLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLK bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MYLK, DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK, OTTHUMP00000180642, myosin, light polypeptide kinase, smooth muscle myosin light chain kinase |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 338037 |
| Storage | 2-8°C |
| Background | Myosin light chain kinase, smooth muscle (MYLK), a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. MYLK encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. |
| Supplier | Abbkine |
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