Bovine Phosphatidylinositide phosphatase SAC1 (SACM1L) ELISA Kit
| Product Name | Bovine Phosphatidylinositide phosphatase SAC1 (SACM1L) ELISA Kit |
|---|---|
| Description | Bovine Phosphatidylinositide phosphatase SAC1 (SACM1L) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine SACM1L. No significant cross-reactivity or interference between Bovine SACM1L and analogues was observed. SAC1, a 587-amino acid protein with 34% sequence identity with the yeast RSD1 (recessive suppressor of secretory defect) protein. RT-PCR detected expression of SAC1 in heart, brain, lung, liver, kidney, pancreas, testis, and ovary, and less abundant expression in skeletal muscle and spleen. mouse Sac1 which encodes a protein sharing 95% sequence identity with human SAC1. Both contain 2 transmembrane regions and a leucine zipper pattern. Both are predicted to be cytoplasmic proteins in the endoplasmic reticulum. Northern blot analysis in mouse showed high expression of a 4-kb transcript in brain, lung, liver, and kidney. A lower level of expression was seen in spleen and skeletal muscle, and no expression was observed in heart or testis. This Bovine Phosphatidylinositide phosphatase SAC1 (SACM1L) ELISA Kit employs a two-site sandwich ELISA to quantitate SACM1L in samples. An antibody specific for SACM1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySACM1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SACM1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SACM1L bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SACM1L, DKFZp686A0231, KIAA0851, SAC1, phosphatidylinositide phosphatase SAC1, suppressor of actin 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 530577 |
| Storage | 2-8°C |
| Background | SAC1, a 587-amino acid protein with 34% sequence identity with the yeast RSD1 (recessive suppressor of secretory defect) protein. RT-PCR detected expression of SAC1 in heart, brain, lung, liver, kidney, pancreas, testis, and ovary, and less abundant expression in skeletal muscle and spleen. mouse Sac1 which encodes a protein sharing 95% sequence identity with human SAC1. Both contain 2 transmembrane regions and a leucine zipper pattern. Both are predicted to be cytoplasmic proteins in the endoplasmic reticulum. Northern blot analysis in mouse showed high expression of a 4-kb transcript in brain, lung, liver, and kidney. A lower level of expression was seen in spleen and skeletal muscle, and no expression was observed in heart or testis. |
| Supplier | Abbkine |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
