| Product Name | Bovine Prolyl endopeptidase (PREP) ELISA Kit |
|---|---|
| Description | Bovine Prolyl endopeptidase (PREP) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PREP. No significant cross-reactivity or interference between Bovine PREP and analogues was observed. Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene.Prolyl endopeptidase is a large cytosolic enzyme that belongs to a distinct class of serine peptidases. It was first described in the cytosol of rabbit brain as an oligopeptidase, which degrades the nonapeptide bradykinin at the Pro-Phe bond.[5] The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimulating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. PREP cleaves peptide bonds at the C-terminal side of proline residues. Its activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. Prolyl endopeptidases are involved in the maturation and degradation of peptide hormones and neuropeptides. This Bovine Prolyl endopeptidase (PREP) ELISA Kit employs a two-site sandwich ELISA to quantitate PREP in samples. An antibody specific for PREP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PREP, RP3-355L5.1, MGC16060, PE, PEP, dJ355L5.1 (prolyl endopeptidase), post-proline cleaving enzyme, prolyl oligopeptidase |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 286818 |
| Storage | 2-8°C |
| Background |
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene.Prolyl endopeptidase is a large cytosolic enzyme that belongs to a distinct class of serine peptidases. It was first described in the cytosol of rabbit brain as an oligopeptidase, which degrades the nonapeptide bradykinin at the Pro-Phe bond.[5] The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimulating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. PREP cleaves peptide bonds at the C-terminal side of proline residues. Its activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. Prolyl endopeptidases are involved in the maturation and degradation of peptide hormones and neuropeptides. |
| Supplier | Abbkine |
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