| Product Name | Bovine Protamine-2 (PRM2) ELISA Kit |
|---|---|
| Description | Bovine Protamine-2 (PRM2) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PRM2. No significant cross-reactivity or interference between Bovine PRM2 and analogues was observed. Presumably PRM2 is located on human chromosome 16, close to PRM1: in the mouse the corresponding 2 loci are closely linked, and in the Chinese hamster, probes specific for the 2 protamines hybridize to the same restriction fragments after digestion of hamster genomic DNA with any of 5 different restriction endonucleases (Reeves et al., 1987, 1989). The tight linkage may be of functional significance since PRM1 and PRM2 are among the limited number of genes known to be expressed postmeiotically in haploid cells. In mouse, only one P2 protamine has been detected, whereas in man, the P2 protamine family is represented by 3 protamines, HP2, HP3 and HP4, whose amino acid sequences are identical except at their amino terminal ends . This Bovine Protamine-2 (PRM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM2 in samples. An antibody specific for PRM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PRM2, CT94.2, FLJ27447, Sperm protamine P2, cancer/testis antigen family 94, member 2 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 281424 |
| Storage | 2-8°C |
| Background |
Presumably PRM2 is located on human chromosome 16, close to PRM1: in the mouse the corresponding 2 loci are closely linked, and in the Chinese hamster, probes specific for the 2 protamines hybridize to the same restriction fragments after digestion of hamster genomic DNA with any of 5 different restriction endonucleases (Reeves et al., 1987, 1989). The tight linkage may be of functional significance since PRM1 and PRM2 are among the limited number of genes known to be expressed postmeiotically in haploid cells. In mouse, only one P2 protamine has been detected, whereas in man, the P2 protamine family is represented by 3 protamines, HP2, HP3 and HP4, whose amino acid sequences are identical except at their amino terminal ends . |
| Supplier | Abbkine |
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