Bovine Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit
| Product Name | Bovine Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit |
|---|---|
| Description | Bovine Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PPP1R1B. No significant cross-reactivity or interference between Bovine PPP1R1B and analogues was observed. Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders.In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1. This Bovine Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1R1B in samples. An antibody specific for PPP1R1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1R1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1R1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R1B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PPP1R1B, DARPP-32, DARPP32, FLJ20940, OTTHUMP00000164276, dopamine and cAMP regulated phosphoprotein, dopamine and cAMP-regulated neuronal phosphoprotein 32, protein phosphatase 1, regulatory (inhibitor) subuni |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 282459 |
| Storage | 2-8°C |
| Background | Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders.In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1. |
| Supplier | Abbkine |
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