| Product Name | Bovine Protein phosphatase 1A (PPM1A) ELISA Kit |
|---|---|
| Description | Bovine Protein phosphatase 1A (PPM1A) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PPM1A. No significant cross-reactivity or interference between Bovine PPM1A and analogues was observed. PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described. This Bovine Protein phosphatase 1A (PPM1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PPM1A in samples. An antibody specific for PPM1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPM1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPM1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPM1A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PPM1A, FLJ42306, MGC9201, PP2C-ALPHA, PP2CA, PP2Calpha, phosphatase 2C alpha, protein phosphatase 1A, protein phosphatase 2C alpha isoform |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 281994 |
| Storage | 2-8°C |
| Background | PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described. |
| Supplier | Abbkine |
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