Bovine Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit
| Product Name | Bovine Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit |
|---|---|
| Description | Bovine Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PRDM6. No significant cross-reactivity or interference between Bovine PRDM6 and analogues was observed. PRDM6, is enriched in flk1(+) hematovascular precursor cells using a microarray-based approach. PRDM6 is also detected in embryonic and adult-derived endothelial cell lines. PRDM6 is co-localized with histone H4 and methylates H4-K20 (but not H3) in vitro and in vivo. Overexpression of PRDM6 in mouse embryonic endothelial cells induces apoptosis by activating caspase-3 and inducing G1 arrest. PRDM6 inhibits cell proliferation as determined by BrdU incorporation in endothelial cells, but not in rat aortic smooth muscle cells. PRDM6 is expressed by vascular precursors, has differential effects in endothelial cells and smooth muscle cells, and may play a role in vascular precursor differentiation and survival by modulating local chromatin-remodeling activity within hematovascular subpopulations during development. This Bovine Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM6 in samples. An antibody specific for PRDM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM6 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PRDM6, PR-domain zinc finger protein 6 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 519857 |
| Storage | 2-8°C |
| Background | PRDM6, is enriched in flk1(+) hematovascular precursor cells using a microarray-based approach. PRDM6 is also detected in embryonic and adult-derived endothelial cell lines. PRDM6 is co-localized with histone H4 and methylates H4-K20 (but not H3) in vitro and in vivo. Overexpression of PRDM6 in mouse embryonic endothelial cells induces apoptosis by activating caspase-3 and inducing G1 arrest. PRDM6 inhibits cell proliferation as determined by BrdU incorporation in endothelial cells, but not in rat aortic smooth muscle cells. PRDM6 is expressed by vascular precursors, has differential effects in endothelial cells and smooth muscle cells, and may play a role in vascular precursor differentiation and survival by modulating local chromatin-remodeling activity within hematovascular subpopulations during development. |
| Supplier | Abbkine |
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