Bovine Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit
| Product Name | Bovine Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit |
|---|---|
| Description | Bovine Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine TIMP4. No significant cross-reactivity or interference between Bovine TIMP4 and analogues was observed. Leco et al. (1997) cloned cDNAs encoding mouse Timp4. The predicted Timp4 protein contains the hallmarks of TIMP proteins, including 12 conserved cysteine residues and 2 short conserved sequence motifs. Northern blot analysis of adult mouse tissues detected Timp4 expression in brain, heart, ovary, and skeletal muscle. Olson et al., 1998 determined that the TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. They demonstrated a high degree of conservation of gene structure in the TIMP family. By FISH, Olson et al., 1998 mapped the TIMP4 gene to chromosome 3p25. By interspecific backcross analysis, they mapped the mouse Timp4 gene to chromosome 6 in a region of syntenic homology with human chromosome 3. This Bovine Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP4 in samples. An antibody specific for TIMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | TIMP4, Tissue inhibitor of metalloproteinase 4 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 317694 |
| Storage | 2-8°C |
| Background |
Leco et al. (1997) cloned cDNAs encoding mouse Timp4. The predicted Timp4 protein contains the hallmarks of TIMP proteins, including 12 conserved cysteine residues and 2 short conserved sequence motifs. Northern blot analysis of adult mouse tissues detected Timp4 expression in brain, heart, ovary, and skeletal muscle. Olson et al., 1998 determined that the TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. They demonstrated a high degree of conservation of gene structure in the TIMP family. By FISH, Olson et al., 1998 mapped the TIMP4 gene to chromosome 3p25. By interspecific backcross analysis, they mapped the mouse Timp4 gene to chromosome 6 in a region of syntenic homology with human chromosome 3. |
| Supplier | Abbkine |
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