| Product Name | Bovine Toll-like receptor 3 (TLR3) ELISA Kit |
|---|---|
| Description | Bovine Toll-like receptor 3 (TLR3) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine TLR3. No significant cross-reactivity or interference between Bovine TLR3 and analogues was observed. TLR 3 is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. Discovered in 2001,TLR3 recognizes double-stranded RNA, a form of genetic information carried by some viruses such as reoviruses. Upon recognition, TLR 3 induces the activation of NF-kB to increase production of type I interferons which signal other cells to increase their antiviral defenses. Double-stranded RNA is also recognised by the cytoplasmic receptors RIG-I and MDA-5.The structure of TLR3 was reported in June 2005 by researchers at The Scripps Research Institute. TLR3 forms a large horseshoe shape that contacts with a neighboring horseshoe, forming a "dimer" of two horseshoes. This Bovine Toll-like receptor 3 (TLR3) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR3 in samples. An antibody specific for TLR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | TLR3, CD283, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 281535 |
| Storage | 2-8°C |
| Background |
TLR 3 is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. Discovered in 2001,TLR3 recognizes double-stranded RNA, a form of genetic information carried by some viruses such as reoviruses. Upon recognition, TLR 3 induces the activation of NF-kB to increase production of type I interferons which signal other cells to increase their antiviral defenses. Double-stranded RNA is also recognised by the cytoplasmic receptors RIG-I and MDA-5.The structure of TLR3 was reported in June 2005 by researchers at The Scripps Research Institute. TLR3 forms a large horseshoe shape that contacts with a neighboring horseshoe, forming a "dimer" of two horseshoes. |
| Supplier | Abbkine |
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