| Product Name | Canine Dihydrotestosterone (DHT) ELISA Kit |
|---|---|
| Description | Dihydrotestosterone (DHT), or 5a-dihydrotestosterone (5a-DHT), also known as 5a-androstan-17b-ol-3-one, is an endogenous androgen sex steroid and hormone. The enzyme 5a-reductase catalyzes the formation of DHT from testosterone in certain tissues including the prostate gland, seminal vesicles, epididymides, skin, hair follicles, liver, and brain. This enzyme mediates reduction of the C4-5 double bond of testosterone. Relative to testosterone, DHT is considerably more potent as an agonist of the androgen receptor (AR). Canine Dihydrotestosterone (DHT) ELISA Kit employs a two-site sandwich ELISA to quantitate DHT in samples. An antibody specific for DHT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHT present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Canine DHT detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of DHT bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DHT |
| Method | Sandwich ELISA |
| Detection Range | 3 nmol/L-48 nmol/L |
| Sensitivity | 0.1 nmol/L |
| Reactivity | Canine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Dihydrotestosterone (DHT), or 5a-dihydrotestosterone (5a-DHT), also known as 5a-androstan-17b-ol-3-one, is an endogenous androgen sex steroid and hormone. The enzyme 5a-reductase catalyzes the formation of DHT from testosterone in certain tissues including the prostate gland, seminal vesicles, epididymides, skin, hair follicles, liver, and brain. This enzyme mediates reduction of the C4-5 double bond of testosterone. Relative to testosterone, DHT is considerably more potent as an agonist of the androgen receptor (AR). |
| Supplier | Abbkine |
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