Canine Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit
| Product Name | Canine Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit |
|---|---|
| Description | Canine Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit has high sensitivity and excellent specificity for detection of Canine VEGFA. No significant cross-reactivity or interference between Canine VEGFA and analogues was observed. This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. This Canine Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFA in samples. An antibody specific for VEGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFA bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Canine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 403802 |
| Storage | 2-8°C |
| Background | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. |
| Supplier | Abbkine |
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