| Product Name | Canine Vitamin A (VA) ELISA Kit |
|---|---|
| Description | Canine Vitamin A (VA) ELISA Kit has high sensitivity and excellent specificity for detection of Canine VA. No significant cross-reactivity or interference between Canine VA and analogues was observed. Vitamin A is a group of unsaturated nutritional organic compounds that includes retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably beta-carotene). Vitamin A has multiple functions: it is important for growth and development, for the maintenance of the immune system and good vision. Vitamin A is needed by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, the light-absorbing molecule[4] necessary for both low-light (scotopic vision) and color vision.[5] Vitamin A also functions in a very different role as retinoic acid (an irreversibly oxidized form of retinol), which is an important hormone-like growth factor for epithelial and other cells. This Canine Vitamin A (VA) ELISA Kit employs a two-site sandwich ELISA to quantitate VA in samples. An antibody specific for VA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VA bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | VA |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Canine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Vitamin A is a group of unsaturated nutritional organic compounds that includes retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably beta-carotene). Vitamin A has multiple functions: it is important for growth and development, for the maintenance of the immune system and good vision. Vitamin A is needed by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, the light-absorbing molecule[4] necessary for both low-light (scotopic vision) and color vision.[5] Vitamin A also functions in a very different role as retinoic acid (an irreversibly oxidized form of retinol), which is an important hormone-like growth factor for epithelial and other cells. |
| Supplier | Abbkine |
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